Abstract
Newcastle disease virus (NDV) belongs to genus Avulavirus and family Paramyxoviridae. There are thirteen serotypes named APMV-I (Avian Paramyxovirus-I) to APMV-13 of which NDV has been designated as APMV-1. The disease has been reported worldwide affecting both domestic and wild avian species. Morbidity and mortality rates up to 100% have been reported in cases of unvaccinated flocks. Stringent vaccination schedule is practiced in endemic/disease prone areas in order to prevent the disease. Despite this, NDV outbreaks have been reported even in cases of vaccinated populations. In this study we describe detailed pathological and molecular investigation that were undertaken in an organized poultry farm from Bareilly region, Uttar Pradesh, India, involving layer flocks which succumbed to ND outbreak in spite of following strict vaccination protocol. The mortality rate ranged from 76.80 to 84.41% in different flocks with an average mortality of 79.50%. Necropsied birds had gross lesions suggestive of viscerotropic ND including petechial hemorrhages on the proventricular tips, intestinal lumen with necrotic areas covered with hemorrhages, hemorrhagic cecal tonsils, para-tracheal edema and mottling of spleen. The characteristic histopathological lesions were mainly seen in the blood vessels and lymphoid tissues. Vascular changes characterized by congestion, edema, and hemorrhage were found in majority of the organs. Lymphocytolysis in spleen and cecal tonsils was evident. Immunohistochemical studies revealed positive signals mostly in macrophage and lymphocytes. PCR assay was done to confirm the NDV genome, which revealed an amplicon size of 356 bp. The phylogenetic analysis revealed the resemblance of the present isolate (ADS01) with class II genotype NDV XIIIA. The isolate belonged to velogenic NDV as the Minimum Lethal Dose (MLD) and Mean Death Time (MDT) for the present isolate were 10−8 and 41 h, respectively. Thus this study clearly demonstrates that in spite of strict vaccination regime and biosecurity procedures, ND continues to be rampant. Hence it is important to effectively administer the present vaccine in addition to strains matching to the field isolates to provide longer and optimal protection against spreading of virus by means of reducing the extent of viral shedding.
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References
OIE. Manual of diagnostic tests and vaccines for terrestial animal. 2012. http://www.oie.int/eng/normes/manual
Bulbule NR, Madale DS, Meshram CD, Pardeshi RB, Chawak MM. Virulence of newcastle disease virus and diagnostic challenges. Adv Anim Vet Sci. 2015;3:14–21.
Cattoli G, Susta L, Terregino C, Brown C. Newcastle disease: a review of field recognition and current methods of laboratory detection. J Vet Diagn Investig. 2011;23:637–56.
van Boven M, Bouma A, Fabri TH, Katsma E, Hartog L, Koch G. Herd immunity to Newcastle disease virus in poultry by vaccination. Avian Pathol. 2008;37:1–5.
Luna LG. Manual of histologic staining methods of the armed forces institute of pathology. New York: Mc Graw Hill; 1968.
Swanson P. Microwave antigen retrieval in citrate buffer. Lab. Med. 1994;25:520–2.
Jindal N, Chander Y, Primus A, Redig P, Goyal S. Isolation and molecular characterization of Newcastle disease viruses from raptors. Avian Pathol. 2010;39:441–5.
Jaganathan S, Ooi PT, Phang LY, Allaudin ZNB, Yip LS, Choo PY, Lim BK, Lemiere S, Audonnet JC. Observation of risk factors, clinical manifestations and genetic characterization of recent Newcastle Disease Virus outbreak in West Malaysia. BMC Vet Res. 2015;11:219.
Kimura M. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol. 1980;16:111–20.
Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution. 1985;39:783–91.
Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol. 2016;33:1870–4.
Desingu P, Singh S, Dhama K, Karthik K, Vinodh Kumar O, Malik Y. Phylogenetic analysis of Newcastle disease virus isolates occurring in India during 1989–2013. Virus Dis. 2016;27:203–6.
Bilal ES, Elnasri IM, Alhassan AM, Khalifa KA, Elhag JI, Ahmed SO. Biological pathotyping of Newcastle disease viruses in Sudan 2008–2013. J Vet Med. 2014;2014:209357. https://doi.org/10.1155/2014/209357.
Abdel-Glil MY, Mor SK, Sharafeldin TA, Porter RE, Goyal SM. Detection and characterization of Newcastle disease virus in formalin-fixed, paraffin-embedded tissues from commercial broilers in Egypt. Avian Dis. 2014;58(1):118–23.
Alexander DJ. Newcastle disease. Gordon memorial lecture. Br Poult Sci. 2001;42:5–22.
Alexander DJ, Senne DA. Newcastle disease and other avian paramyxoviruses. In: Dufour-Zavala L, Senne DA, Glisson JR, et al., editors. A laboratory manual for the isolation, identification and characterization of avian pathogens. 5th ed. Athens: American Association of Avian Pathologists; 2008. p. 135–41.
Kommers GD, King DJ, Seal BS, Brown CC. Pathogenesis of chicken-passaged Newcastle disease viruses isolated from chickens and wild and exotic birds. Avian Dis. 2003;47:319–29.
Susta L, Miller PJ, Afonso CL, Brown CC. Clinicopathological characterization in poultry of three strains of Newcastle disease virus isolated from recent outbreaks. Vet Pathol. 2011;48(2):349–60.
Jakhesara S, Prasad V, Pal J, Jhala M, Prajapati K, Joshi C. Pathotypic and sequence characterization of newcastle disease viruses from vaccinated chickens reveals circulation of genotype II, IV and XIII and in India. Transbound Emerg Dis. 2014;63:523–39.
Khorajiya J, Pandey S, Ghodasara P, Joshi B, Prajapati K, Ghodasara D, Mathakiya R. Patho-epidemiological study on Genotype-XIII Newcastle disease virus infection in commercial vaccinated layer farms. Vet World. 2015;8:372–81.
Gowthaman V, Singh S, Dhama K, Desingu P, Kumar A, Malik Y, Munir M. Isolation and characterization of genotype XIII Newcastle disease virus from Emu in India. Virus Dis. 2016;27:315–8.
Alexander DJ, Elizabeth WA, Chad MF. The long view: a selective review of 40 years of Newcastle disease research. Avian Pathol. 2012;41:329–35.
Nakamura K, Ohtsu N, Nakamura T, Yamamoto Y, Yamada M, Mase M, Imai K. Pathologic and immunohistochemical studies of Newcastle disease (ND) in broiler chickens vaccinated with ND: severe non-purulent encephalitis and necrotizing pancreatitis. Vet Pathol. 2008;45:928–33.
Perozo F, Marcano R, Afonso CL. Biological and phylogenetic characterization of a genotype VII Newcastle disease virus from Venezuela: efficacy of field vaccination. J Clin Microbiol. 2012;50:1204–8.
Kapczynski DR, King DJ. Protection of chickens against overt clinical disease and determination of viral shedding following vaccination with commercially available Newcastle disease virus vaccines upon challenge with highly virulent virus from the California 2002 exotic Newcastle disease outbreak. Vaccine. 2005;23:3424–33.
Miller PJ, King DJ, Afonso CL, Suarez DL. Antigenic differences among Newcastle disease virus strains of different genotypes used in vaccine formulation affect viral shedding after a virulent challenge. Vaccine. 2007;25:7238–46.
Alexander DJ, Manvell RJ, Banks J, Collins MS, Parsons G, Cox B, Frost KM, Speidel EC, Ashman S, Aldous EW. Experimental assessment of the pathogenicity of the Newcastle disease viruses from outbreaks in Great Britian in 1997 for chickens and turkeys and the protection afforded by vaccination. Avian Pathol. 1999;28:501–12.
King DJ. Avian paramyxovirus type 1 from pigeons: isolate characterization and pathogenicity after chicken or embryo passage of selected isolates. Avian Dis. 1996;40:707–14.
Hu S, Ma H, Wu Y, Liu W, Wang X, Liu Y, Liu X. A vaccine candidate of attenuated genotype VII Newcastle disease virus generated by reverse genetics. Vaccine. 2009;27(6):904–10.
Miller PJ, Estevez C, Yu Q, Suarez DL, King DJ. Comparison of viral shedding following vaccination with inactivated and live Newcastle disease vaccines formulated with wild-type and recombinant viruses. Avian Dis. 2009;53:39–49.
Rauw F, Gardin Y, Palya V, Anbari S, Lemaire S, Boschmans M, van den Berg T, Lambrecht B. Improved vaccination against Newcastle disease by an in ovo recombinant HVT-ND combined with an adjuvanted live vaccine at day-old. Vaccine. 2010;28:823–33.
Palya V, Kiss I, Tatar-Kis T, Mato T, Felfoldi B, Gardin Y. Advancement in vaccination against Newcastle disease: recombinant HVT NDV provides high clinical protection and reduces challenge virus shedding with the absence of vaccine reactions. Avian Dis. 2012;56:282–7.
Roohani K, Tan S, Yeap S, Ideris A, Bejo M, Omar A. Characterisation of genotype VII Newcastle disease virus (NDV) isolated from NDV vaccinated chickens, and the efficacy of LaSota and recombinant genotype VII vaccines against challenge with velogenic NDV. J Vet Sci. 2015;16:447.
Bwala DG, Abolnik C, van Wyk A, Cornelius E, Bisschop SP. Efficacy of a genotype 2 Newcastle disease vaccine (Avinew) against challenge with highly virulent genotypes 5 and 3d. J S Afr Vet Assoc. 2009;80:174–8.
Liu XF, Wan HQ, Ni XX, Wu YT, Liu WB. Pathotypical and genotypical characterization of strains of Newcastle disease virus isolated from outbreaks in chicken and goose flocks in some regions of China during 1985–2001. 2003. Arch Virol. 2003;148:1387–403.
Dortmans J, Peeters B, Koch G. Newcastle disease virus outbreaks: vaccine mismatch or inadequate application? Vet Microbiol. 2012;160:17–22.
Susta L, Jones M, Cattoli G, Cardenas-Garcia S, Miller P, Brown C, Afonso C. Pathologic characterization of genotypes XIV and XVII Newcastle disease viruses and efficacy of classical vaccination on specific pathogen-free birds. Vet Pathol. 2014;52:120–31.
Taylor TL, Miller PJ, Olivier TL, Montiel E, Cardenas Garcia S, Dimitrov KM, Williams-Coplin D, Afonso CL. Repeated challenge with virulent Newcastle disease virus does not decrease the efficacy of vaccines. Avian Dis. 2017;61(2):245–9.
Jeon WJ, Lee EK, Lee YJ, Jeong OM, Kim YJ, Kwon JH, Choi KS. Protective efficacy of commercial inactivated Newcastle disease virus vaccines in chickens against a recent Korean epizootic strain. J Vet Sci. 2008;9:295–300.
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We thank the Director, ICAR-Indian Veterinary Research Institute for providing necessary facilities and support.
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Mariappan, A.K., Munusamy, P., Kumar, D. et al. Pathological and molecular investigation of velogenic viscerotropic Newcastle disease outbreak in a vaccinated chicken flocks. VirusDis. 29, 180–191 (2018). https://doi.org/10.1007/s13337-018-0445-5
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DOI: https://doi.org/10.1007/s13337-018-0445-5